发信人: moso (野鬼), 信区: Biology
标 题: Re: Northern vs RT-PCR
发信站: Unknown Space - 未名空间 (Thu Mar 4 19:18:17 2004), 转信

I guess, you may misunderstand what your director's idea.
He may wanna know before identification if the transcription level has
significant difference between the WT and mutants. For people always care
much a functional mutation rather than a sequence mutation itself. And most
researchers like to start from a functional change. Your idea could be
classified into reverse genetical method. It is ok, but not often be used in
a single gene case.
【 在 beebee (^_^) 的大作中提到: 】
: 老板要看一个基因的mutation, 我说做个RT-PCR,再sequence一下，在和正常的比一下不
: 就知道有没有muations 了吗？老板说，不对。应该先做NORTHERN INSTEAD OF RT-PCR ,
: 然后再，巴拉，巴拉。。。还要我去思考。问了别人，别人说这是初级问题，不告诉我，
: 让我自己想。


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