发信人: justsososo (justsososo), 信区: Biology
标 题: Re: recombinant蛋白质表达问题
发信站: Unknown Space - 未名空间 (Fri Apr 30 18:55:48 2004) WWW-POST

Yes. Not only BL21codon plus, but almost all BL21s except BL21(DE3)pLysS/E are
leaky. pLysS/E encode enzyme that degrade T7 polymerase to avoid leaky.

In your case with many rare Arg codons, you have to use codon plus. So, it is
importment to avoid leaky? otherwise, just express with leaky if it does no
harm.

【 在 arabidopsis (moon) 的大作中提到: 】
: I tried this strain and cannot get my protein induced. There are lots of
: rare Arg codons in my DNA sequence. That's why I chose BL21 codon plus.
: BL21 codon plus usually leaky?
:
: 【 在 justsososo (justsososo) 的大作中提到: 】
: : If you want to avoid leaky, use cell line BL21(DE3)pLysS.
: : 【 在 arabidopsis (moon) 的大作中提到: 】
: : : I'm using BL21 codon plus.
:
:


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