发信人: mfelegans (celegans), 信区: Biology
标 题: Re: 3' UTR
发信站: Unknown Space - 未名空间 (Wed May 5 12:42:45 2004) WWW-POST
In C.elegans, all kinds of expression vectors are available. N-terminalor
C-terminal fused, cytoplasm located or nuclear located. Usually, we use the
same 3'UTR. Sometime we will use the gene specific 3'UTR in order to know the
accurate expression pattern of that gene.
【 在 clonist (Epi) 的大作中提到: 】
: u confuse me. if you cut your gene at its 3'UTR then ligate it
: into the MCS of target vector it should be fine. if you insert
: your gene into the site at the 3'UTR of target vector it could
: have problem.
:
: 【 在 Cathleen (蛹-@-化蝶) 的大作中提到: 】
: : too many of the RE sites exist inside that gene, I comprimised to use a RE
: : that doesn't not inside the gene, but upstream the 3'UTR, surprisingly, I
: : detected the expression by western.
: : here I meet the same problem with another insertion, don't know if I'd be
that
: : lucky or not.
: : 【 在 clonist (Epi) 的大作中提到: 】
: : : in most cases i don't think that gene will even express if the
: : : 3'UTR is missing. many regulatory factors bind the 3'UTR to
: : : function let alone those polyA stuff
: : the
:
:
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※ 来源:.Unknown Space - 未名空间 mitbbs.com.[FROM: 129.130.]
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