发信人: moso (野鬼), 信区: Biology
标 题: Re: 问一个AMPLIFY GENOMIC DNA 的问题
发信站: Unknown Space - 未名空间 (Fri May 14 18:02:01 2004), 转信

Yes, PCR method can be used for some cases.
what I am saying is that you have to consider the fidelity of those methods. If
fidelity could be sacrificed, PCR of cz could be used to amplify the DNA. Thats
why I was saying it depends on your purpose of amplification.
You must understand if the fidelity can be sacrificed before deciding to use
PCR. Well, it is possible High F PCR could overcome the problem.
I did not say that PCR method can not be used.
【 在 magie (*^*) 的大作中提到: 】
: OK, 明白了, 多谢
: 不过要是有兴趣, 请看看这个, 好象还是能做的, 只是我不清楚细节.
: http://micro.nwfsc.noaa.gov/protocols/methods/DNA-PCRMethodsDocs/10-2002/10.21
: .02.5889.html
: 【 在 moso (野鬼) 的大作中提到: 】
: : Whatever method will be used, the fidelity is the most concern. To that
: point
: : of view, PCR method is not recommended. Well, it finally depends on your
: : purpose of the amplification, that means how much the fidelity would be your
: : concern.
: : By the way, what is the basis of your DNA pool?
: you
: knows


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