发信人: Ras (很颓的猪。), 信区: Biology
标 题: Re: how to make quiescent cells?
发信站: Unknown Space - 未名空间 (Thu May 27 15:56:13 2004), 转信


yes, in most cases serum starvation should do fine for your case.

Be careful with the serum starvation time though. Sometime you need
to starve the cells really long (till they start to die) to achieve
the maximum quienscence. Sometimes you only need to start 24 hours or less
to obtained your desired result. Start with a time course test will surely
help.

【 在 bewithu (x-fire in the sea) 的大作中提到: 】
: Thank u for ur reply, Ras.
: In my case, a nuclear receptor is focused on. Others reported that the receptor
: expressed right after transfections locates in both the cytosol and the nuclei,
: and that its phosphorylation (by p38 or other unidentified kinase) could motivate
: the receptor translocate into the nuclei.
: Thus I feel confused whether phosphorylation is necessary of the translocation,
: because part already locate in the nuclei before the activation of p38. So I wana
: reduce or silence (if possilbe) the activity of protein kinase before transfection,
: to see whether some proteins could accumualte in the nuclei.
: And do you think that serum deprivation could reduce the activity of kinase to
: very low level?
: thank u for ur time
: 【 在 Ras (很颓的猪。) 的大作中提到: 】
: : what signal are u looking at later on?


--
一年老一年，一日没一日，
一秋又一秋，一辈催一辈，
一聚一离别，一喜一伤悲。
一榻一身卧，一生一梦里。
寻一夥相识，他一会咱一会，都一般相知，
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