发信人: forrestgump (阿甘), 信区: Biology
标 题: Re: 蛋白纯化的脱盐问题
发信站: Unknown Space - 未名空间 (Fri Jun 18 12:41:36 2004) WWW-POST

desalting column is much faster, but sometimes proteins bind to it
non-specifically and you may lose some of your sample by that. sometimes,
proteins get denatured because the buffer change is too sudden, not like
dialysis that is slow and "tender". plus, carry over is almost inevitable on
the column, and a 2nd run is sometimes necessary.
dialysis on the other hand, has its disadvantage too. most of all, it takes
way too long (O/N is routine), during which the protein may not be happy.
second, it dilutes ur sample to quite a bit (even more so than the column).
u can read about this on those "current protocol of ..." books.
anyway, if you have plenty of samples, i would go for the column.

【 在 comeandgo (春困秋乏夏打盹) 的大作中提到: 】
: 用 5mM NH3HCO3 透析， 冻干时NH3HCO3也自动挥发
: 【 在 capry (IA) 的大作中提到: 】
: : Desalting column or dialysis bag, 哪个更彻底一些？有相应的文献资料吗？
: :
: : 多谢
: :
: :
:

--
LIfe is like a box of chocolate.
You'll never know what you are gonna get.

※ 来源:．Unknown Space - 未名空间 mitbbs.com．[FROM: 170.223.]