发信人: tops (digging), 信区: Biology
标 题: Re: PCR primer question
发信站: Unknown Space - 未名空间 (Fri Jul 2 02:30:27 2004), 站内信件

I think different softwares score primers differently. But i don't think
they can be too much off each other.

Primers are dirt cheap. Why don't you design 2 pairs out of a software
and do an annealing temperature optimization? Or maybe run touch-down PCR.

RE map is restriction enyme map. You cut your PCR products with "RE"
and run on a gel to see if the cut produces correct DNA fragment bands.


【 在 tootootootoo (tutu) 的大作中提到: 】
: I do not have experience on PCR, but now have to do real time PCR for my
: project.
: Do different primer design softwares give different score to the same primer
: pair? How do we decide which one we should believe? I evaluate a primer pair
: published in literature(designed with primer express) with beacon design 2,
: however the evaluation showed this primer was poor.
: I heard to confirm the pcr product using RE map, what is RE map?


--
实验总是做不完的,
老婆可只有一个...

※ 来源:．Unknown Space - 未名空间 mitbbs.com．[FROM: 204.52.]