发信人: leohawk (leohawk), 信区: Biology
标 题: Re: 免疫荧光对照有染色怎搞？
发信站: Unknown Space - 未名空间 (Tue Jul 13 09:49:14 2004), 转信

let me tell you the best way ( I think ):

preabsorption

which means you first incubate your 2nd antibody with your control for several
hours, then use the pre-incubated antibody on your real sample.

it usually gives great results.

hope this helps.

【 在 bewithu (x-fire in the sea) 的大作中提到: 】
: The control is the one without 1st Ab incubation.
: The results show flurescence(FITC) in control cells, which is weaker than
: that in non-control cells. I used 3*5 min washing in TBS. Should I use longer
: time of washing or add Trition to TBS?
: Thank u for ur time


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