发信人: kiddingyou (李寻欢没刀了), 信区: Biology
标 题: Re: ask a question about mass spectrometry
发信站: Unknown Space - 未名空间 (Tue Jul 13 15:55:58 2004) WWW-POST

actually, it's a quite popular the hot topic right now in MS field. I'm gonna
do similiar stuff. I'm reading the relating papers. As far as I know, there
are even non-gel based method to do this kind of stuff. You ignored how
powerful mass spectrometer can do nowadays. There are tons of ways to
identified phosphoproteomics and one of the simple one is to detech whether
there's a ion that lost 98 m/z or 49 for doubly-charged or 33 for
triply-charged ions. Anyway, read some reviews on phosphoproteomics. You will
have a general idea. It should be that hard to do theoritically.
【 在 comeandgo (春困秋乏夏打盹) 的大作中提到: 】
: thank you for you guys's suggestion. I do know well that this protein can be
: phosphorylated at some specific tyrosine. My project is to determine other
: potential tyrosine that can be phosphorylated and serve as signaling protein
: binding site as well. my primilinary exp indicate one specific tyrosine may
be
: the target. I am thinking about raising anti-serum against this potential
: tyrosyl phosphorylated segment so that I can do IP ans WB to detect its
: phosphorylation status in vivo. but since effectiveness of anti-serum can
: never be garanteed, so I am wondering if other alternate approach can be
: employed, such as MS.
: by the way, do you guys know any company is good at raising phospho-specific
: antibody?
: 【 在 hooligan (月是故乡明) 的大作中提到: 】
: : This approach is also good, but it is much more troublesome
: : than probing the purified protein with pY antibody.
: :
: : 【 在 yxk (proxy2.ytht.net) 的大作中提到: 】
: : : 用能识别phospho-tyrosine antibody拉下来再用MS鉴定是不是你要的蛋白
: : : 【 在 comeandgo (春困秋乏夏打盹) 的大作中提到: 】
: : : : then any suggestion?
: : : : how
: : : : and
: :
: :
:

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