发信人: hooligan (月是故乡明), 信区: Biology
标 题: Re: ask a question about mass spectrometry
发信站: Unknown Space - 未名空间 (Tue Jul 13 22:31:11 2004), 站内信件

If you take a pY peptide and try this, you will not be able to
see the loss of phosphate group easily. The phosphate group on
the Tyr residue is really stable. However, this phenomenon is
true for pT and pS peptides because they are much less stable.
Mass Spec is really powerful, but it can't do everything, even
though I am a MS person and I really hope it is that powerful.:)

【 在 kiddingyou (李寻欢没刀了) 的大作中提到: 】
: actually, it's a quite popular the hot topic right now in MS field. I'm gonna
: do similiar stuff. I'm reading the relating papers. As far as I know, there
: are even non-gel based method to do this kind of stuff. You ignored how
: powerful mass spectrometer can do nowadays. There are tons of ways to
: identified phosphoproteomics and one of the simple one is to detech whether
: there's a ion that lost 98 m/z or 49 for doubly-charged or 33 for
: triply-charged ions. Anyway, read some reviews on phosphoproteomics. You will
: have a general idea. It should be that hard to do theoritically.
: 【 在 comeandgo (春困秋乏夏打盹) 的大作中提到: 】
: : thank you for you guys's suggestion. I do know well that this protein can be
: : phosphorylated at some specific tyrosine. My project is to determine other
: : potential tyrosine that can be phosphorylated and serve as signaling protein
: : binding site as well. my primilinary exp indicate one specific tyrosine may
: be
: : the target. I am thinking about raising anti-serum against this potential
: : tyrosyl phosphorylated segment so that I can do IP ans WB to detect its
: : phosphorylation status in vivo. but since effectiveness of anti-serum can
: : never be garanteed, so I am wondering if other alternate approach can be
: : employed, such as MS.
: : by the way, do you guys know any company is good at raising phospho-specific
: : antibody?


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