发信人: leohawk (leohawk), 信区: Biology
标 题: Re: 免疫荧光对照有染色怎搞？
发信站: Unknown Space - 未名空间 (Wed Jul 14 18:56:27 2004), 转信

I personally think it really depends on your sample, I think shaking
is not going to help in any way... however, I never risked not shaking.
for your particular case, I would say it may be fine if you don't shake--
if it causes your cell to get lost from the slide.

again, you probably need to test it yourself, like in your next
experiment, include shaking and not shaking, see what difference
does it make.

good luck

【 在 bewithu (x-fire in the sea) 的大作中提到: 】
: Hi, leohawk
: Thank u very much for ur great protocol about 2nd Ab preabsorption. But I
: am still confused about the detailed procedure. Do u generally shake the
: sample (at low speed if) for the preabsorption of 2nd Ab?
: BTW, do u think whether shaking is necessary for the incubation of 1st or 2nd
: Ab if cell-attatched slide is used?
: Being grateful
: Best
: 【 在 leohawk (leohawk) 的大作中提到: 】
: : let me tell you the best way ( I think ):
: : preabsorption
: : which means you first incubate your 2nd antibody with your control for several
: : hours, then use the pre-incubated antibody on your real sample.
: : it usually gives great results.
: : hope this helps.


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