发信人: comeandgo (春困秋乏夏打盹), 信区: Biology
标 题: Re: degraded protein?
发信站: Unknown Space - 未名空间 (Tue Jul 20 18:13:39 2004) WWW-POST

if you do IP, you will see heavy chain around 50kd. If it is straight western
blot of tissue lysate, maybe the 50kd band is heavt chain of antibody from
tissue? also, RTK degradation is quite common thing.
【 在 middlemouse (mikey) 的大作中提到: 】
: But he's got strong band, does not like nonspesific.
:
: 【 在 Oncogene (厨长) 的大作中提到: 】
: : 有没有其它杂带?
: :
: : 另外,转膜也可能是个问题,size太大,效率很低的.
: :
: : 【 在 merben (merben) 的大作中提到: 】
: : : I always get smaller band on western than usally it should be.
: : : Say, TrkA should be 120kd around, I got strong, very good 60 around.
: : : For neurofilament 200, I got good ,strong 50kd band.
: : : What's the problem? i am using reducing sds-page gel.
: : : Really bother me a lot.
: :
: :
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