发信人: jonzhang (清华MM的海鸥乔纳森), 信区: Biology
标 题: Re: follow up Re: asking for idea, thanks a
发信站: Unknown Space - 未名空间 (Sat Sep 18 13:17:59 2004) WWW-POST

I am confused too.

Anyway, two points.
1, If you could decrease the secretion, by which the cytokine works in your
culture sys, it's unlike the RNAi will help you. Coz both reduce protein
expression down to 70-90%.
2, For your knockout mice, there are always some cytokines/growth factor
undiscovered, that may play an important role or compensate (as suggested by
Ras).

One piece of suggestion upon your writing. Please specify the phenomina
consistently, coz I was lost in paragraph two. And what's the drug mentioned
in paragraph one, the Ab?

【 在 ogog (miao) 的大作中提到: 】
: Hi, thank you guys a lot on your responses.
: We actually have done more. The molecule we thought play a role is a
cytokine.
: Antibodies against other cytokines doen't block. If we give the cells this
: cytokine instead of the drug, it just did the same job, maybe even better.
:
: Something against the conclusion is that the knockout mouse still show same
: phenomina. But antibodies against cytokines within the same family can block
: such phenomina.
:
: Weired thing is I designed an experiment to block cytokine secretion but no
: difference. Elisa show the concentration of this cytokine has been reduced
: more than 70%. Do you guys think iRNA is still worth trying? I have never
done
: iRNA before. How well could it block protein expression? I learned from
: commercial website that the efficiency for iRNA is 70~ 90%. Here is my
: concern. How could such little amount of cytokine play so important role.
: Especially because the phenomina it induced is a relatively one, it make me
: feel uncertain to believe it's mediated by a single cytokine instead of
: several cytokines.
:
: What do you guys think?
:
:


--
NEVER, NEVER, NEVER QUIT -- W. CHURCHILL

※ 修改:·jonzhang 於 Sep 18 13:17:59 修改本文·[FROM: 141.213.]
※ 来源:．Unknown Space - 未名空间 mitbbs.com．[FROM: 141.213.]