发信人: John (Peter), 信区: Biology
标 题: Re: 有关免疫组化的问题
发信站: Unknown Space - 未名空间 (Fri Oct 8 14:39:44 2004) WWW-POST

That's not surprising at all. I will be very surprised if it was the
other way around.

Monoclonal antibody is against one specific epitype of antigen. If
it works, it works. If not (for example, under immunohistochemistry
conditions, methanol, paraglutahyde...), it won't. Polyclonal antibody
is against a great variety epitype of the antigens. Some clones of
antibody might only work for western, the others might work for immunohist,
some might work for flow, eta. Very few monoclonal antibodies can
work in all conditions (IP, WB, IM, Immunocytochemistry...), a lot
of polyclonals work for all.

The only problem is polyclonal antibodies will give you background(but
depend on the antibody). The choises are: have a good control, or put
a tag, use another antibody to confirm.


【 在 skzx (roadtoboard) 的大作中提到: 】
: 我正在做组化,遇到一个问题.我有2个一抗,单克隆和多克隆.多克隆给的SIGNAL很好,而
单
: 克隆就不好,几乎没有SIGNAL. 上面人为没有单克隆的DATA让人不舒服.要求用单克隆做
出
: 结果. 我想知道,是不是一定要用单克隆做出DATA ??
:


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