发信人: smallbite (小咬), 信区: Biology
标 题: Re: Questions about culturing of NSC
发信站: Unknown Space - 未名空间 (Wed Dec 1 14:15:33 2004), 转信

It was perfect at first,at least for 4h. But after O/N, almost all died.
I'm 80% sure it's the coating problem.


【 在 bewithu (x-fire in the sea) 的大作中提到: 】
: Thank u for ur suggestion.
: Actually I have been worrying about the process of coating. I coated the plate
: several days ago before use, and the plates were kept in water. I am not sure
: whether I should store the coated plates by adding some water, and just
: letting it dry. But the coated plates are commercially available, and they are
: dry.
: In your case, did the neruons look bad at first, or get unhealthy after
: several hours? Tks
: 【 在 smallbite (小咬) 的大作中提到: 】
: : Just gussing, I never worked with NSC.
: : Maybe the problem is poly-lys coating, use fresh one.
: : At least for primary neuron culture, I found that coating is critical
: : for the survival of cells.
: recently.
: coz I
: pellet
: as
: nearly
: that
: the


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※ 修改:．smallbite 于 Dec 1 14:16:03 修改本文．[FROM: 128.104.]
※ 来源:．Unknown Space - 未名空间 mitbbs.com．[FROM: 128.104.]