发信人: corehistone (magic), 信区: Biology
标 题: Re: 谁从yeast里抽提过plasmid么？
发信站: Unknown Space - 未名空间 (Sun Dec 5 21:58:16 2004) WWW-POST

1. Never expect to isolate a large amount Plasmid DNA from yeast.
2. What I did is:
Incubate lytic enzyme with your yeast for an hour. Then followed the
BioRad kit miniprep protocol, start from lysis solution (equal to solutionII
in classical protocol). I guess kits from other companies are also work.
At the end, elute your DNA from matrix with 20microliter of TE instead of 100
microliter. One microliter of such extract is sufficient for a bacteria
transformation, even though it's CEN plasmid.
Tell me if you have thing unclear about what I said.



【 在 yunyuner (冬 * 眠) 的大作中提到: 】
: 我用zymolyase裂解细胞，然后用miniprep的方法提过好几次了，都不成功。有谁有经
验
: 的么？能不能介绍一下。多谢。
:


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