发信人: royluo ( 罗马情结), 信区: Biology
标 题: Re: Protein precipitation by TCA
发信站: Unknown Space - 未名空间 (Sun Jan 2 23:10:44 2005), 转信


I guess it is due to the residue of ethanol. Use a speed-vac to
dry down the protein pellet completely or incubate the tube with
the sample at 37C for hours.

【 在 canberry (coco) 的大作中提到: 】
: Hello everyone,
: I have a question needing your help. I did protein preciptation by TCA
: today.After I got the pellets and wash them with 75% ethanol, I add the
: loading buffer to the pellets. However the pellets can't dissove into the
: buffer,even after I heated them. So I lost lots of protein.Is anything wrong
: with the procedure? What cause the problem?
: Thanks


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