发信人: John (Peter), 信区: Biology
标 题: Re: 有对线粒体比较熟悉的吗？
发信站: Unknown Space - 未名空间 (Wed Apr 27 20:36:22 2005) WWW-POST

It would be interesting if your parasite will trigger apoptosis. Usually
DNA breakdown is easy to detect 3-6 hours after mitochondrial membrane
potential loss, if your parasite does not have a direct effect on that.

However, there is a big concern. TMRE, derived from the classical potential
dye Rhodamine, will distribute along any potential, of course mitochondrial
membrane potential, but also other kinds of potential, such as plasma membrane
potential. Therefore, during your parasite infection, if there is a change of
plasma membrane potential, it's very likely that you will see a quick drop
of TMRE dye in the cells, although in this case, mitochondria will still have
a higher concentration of TMRE than the cytosol. The easy way to clarify
this is simply reloading your cells with TMRE, or if you are using realtime
confocal microscopy, you can supply some TMRE in the incubation buffer.

【 在 feitian (卡死拍死) 的大作中提到: 】
: start for apoptosis??
:
: 【 在 bewithu (x-fire in the sea) 的大作中提到: 】
: : In my experiment, TMRE was used to pre-label or post-label mitochondria.
In
: : both cases, when cells get infected with one kind of parasite, the
: : TMRE-staining loses (though the cells look pretty healthy).
: :
: : I am really confused abouth this phenomenon. Can someone give me any idea?
: tks
: :
: :
: :
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