发信人: newgard (usanew), 信区: Biology
标 题: Re: 急问: sodium pyruvate in cell culture media
发信站: BBS 未名空间站 (Wed Oct 12 01:35:57 2005)

Sodium pyruvate serves as an additional energy source for cells in culture.
Cells can become "hooked" on sodium pyruvate however, and if it is withdrawn
suddenly from the media, they may experience a short lag in growth.

L-glutamine is essential in cell culture. After deamination, it is used as an
energy source for cultured cells as well as being incorporated into protein
and used in nucleic acid metabolism. L-glutamine undergoes spontaneous
degradation over time in solution.GlutaMAX™ is a stabilized, dipeptide
form of L-glutamine. This direct substitute for L-glutamine is extremely
stable and prevents degradation and toxic ammonia build-up even during long-
term culture.


【 在 sunnyday (艳阳天) 的大作中提到: 】
: 不好意思问个特低级的问题,
: 我在细胞培养上是新手, 平时配培养液时都是用MEM + sodium bicarbonante + Gln+
10%
: serum
: 可是新的细胞说要用DMEM high glucose,然后我就找出来看了一看, 发现不是很明白.
: 瓶子上写的是:
: DMEM, with Glutamax, with high glucose, with 110 mg/L sodium pyruvate, with
: pyridoxine-HCl.
: 厂家是Gibco (Invitrogen), Cat# 10569-010.
: 那个Glutamax我觉得应该就是L-Glutamine, 可是那个sodium pyruvate, with
: pyridoxine-HCl是干什么的?
: ...................



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