发信人: Xerxes (精行仙), 信区: Biology
标 题: Re: GFP signal is weaker in stable cell line??? why
发信站: BBS 未名空间站 (Tue Mar 14 23:41:37 2006), 转信

GFP fluophore is oxidized, and its fluorescence may negatively affect normal
cellular procedure. As a rule, stable line is always the lowest in intensity.
Just my experience:
bombardment > transient by CaCl2/PEG > stable
【 在 horizon (东方之子) 的大作中提到: 】
: Does someone have some opinion: why the GFP signal (I made a stable cell
line
: expressing GFP fusion protein) is weaker in stable cell line comparing to
: transiently transfected cell?
: Thanks!


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