发信人: HENBEN (benben), 信区: Biology
标 题: Re: 同源重组的质粒上的同源片段怎么来的？
发信站: BBS 未名空间站 (Sat Apr 8 22:42:41 2006)

For gene targeting in ES cells, you need a total of at least 5kb sequences (
the longer, the better) in the two homologous arms. The homologous sequence
can be obtained by screening a lambda or BAC genomic library,or by PCR (
sometimes long-PCR).These days it is more fashionable to construct gene
targeting vectors by recombineering using a BAC clone.
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※ 来源:·BBS 未名空间站 http://mitbbs.com·[FROM: 143.111.]