发信人: royluo ( 罗马情结), 信区: Biology
标 题: Re: 大家怎么对付象293这种极易漂浮的细胞的?
发信站: BBS 未名空间站 (Tue Apr 18 17:27:17 2006), 转信
It never worked in my hands at least. I used to do
immunofluorescence on 293 cells. Even very gentle pre-fixation
wash will peel cells off coverslips easily. Polylysine coating
didn't help too much. I finally solved the problem by using
buffer containing Ca2+ and Mg2+.
【 在 windysea (甜心小猪~2006年2月14日,小玫瑰出世了) 的大作中提到: 】
: how about coated (e.g. polylysine) dish?
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※ 来源:·BBS 未名空间站 mitbbs.com·[FROM: 128.112.]
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