发信人: else (where), 信区: Biology
标 题: Re: Kozak sequence
发信站: The unknown SPACE (Tue Jul 1 10:32:33 2003) WWW-POST

【 在 Cathleen (蛹-@-化蝶) 的大作中提到: 】
: I designed it in the primer before the FLAG tag, unfortunately after I
: sequenced the positive clone it turned out one C is missing, now the Kozak
: sequence is "CCAC" which should be "CCACC", will it still work as normal?
:
: Invitrogen really sucks, it gives two point mutations in a 58 length
: primer,fortunately, no frame shift or amino acid change, otherwise I would
: have to start all over.
:

Perfect Kozak is not as short as CCACC.
In your case, if upstream of the CACC is a G instead of A, downstream of ATG
is also a G, it maybe works (less) efficiently; otherwise, I would reconstruct
again.


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