发信人: smileface (笑脸), 信区: Biology
标 题: Re: Northern vs RT-PCR
发信站: Unknown Space - 未名空间 (Thu Mar 4 18:23:50 2004), 站内信件



no,

to study an unknow gene, u need northern first ahead of RT-PCR.

until you have a very specific question about a gene, which
has been studied for a long time.

there are several reasons:
(1) the distribution of a gene
(2) the abundance of a gene
(3) possible isoforms of a gene

such as a simple question is here: the the gene is 3 kb or longer,
u can easily meet a lot of PCR problems to amplify it.








【 在 Aff (阿非) 的大作中提到: 】
: good point
: 【 在 amiga (mi-u-mi) 的大作中提到: 】
: : RT will not help if Northern will not help. You can not prove the point
: : mutation is not generated during RT.
: 一下不
: RT-PCR ,
: 诉我，


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