发信人: moso (野鬼), 信区: Biology
标 题: Re: Q about transfected protein localization
发信站: Unknown Space - 未名空间 (Wed Apr 21 11:01:17 2004), 转信

It looks the sepecificity of subcellular localization was modified. Did you
make any change to the N terminal of the protein? One possibility is the
specific signal pepetides at the N terminal has been modified that led to
the changes of its subcellular localization. Even the tag sequence may make
some changes. Well, sometime, in some case, the modification of subcellular
location may be seen from an overexpression cell line, that's possibly caused
by diffusion of either protein location or detecting signal, like immunoflo-
rescence.
【 在 beebee (^_^) 的大作中提到: 】
: the protein is normally localized in membrane and cytoplasm,
: but if I transfected the same protein in the cells, the protein is localized
: all of the cells. what does that mean? My boss wants me to think about it.
: no idea;-(


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