发信人: bambooo (max dido), 信区: Biology
标 题: Re: dominant negative question
发信站: BBS 未名空间站 (Sat Dec 10 12:25:33 2005)

it is very tricky.

first of all it depends on how well the protein is know. any binding partners
? which part of the protein is
responsible for the binding? if you know delete that part. or if a point
mutation can disrupt the interaction,
do a knock-in.

secondly, if it is in Drosophila and associated with visible phenotype, you
may generate point mutations
with EMS when you definitely need to get one.

third, just make a serial deletion knock out mice, and see what happens.

my 2cents.

【 在 Morphin (莫非-白衣“追鱼”郎) 的大作中提到: 】
: What are the basic strategies to generate a DN form of a know protein?



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