发信人: amador (leopard), 信区: Biology
标 题: Re: 熟悉果蝇的看过来，有个问题要请教
发信站: Unknown Space - 未名空间 (Thu Sep 30 13:52:50 2004) WWW-POST

A gene causing lethality suggests it might be a functionally important. But a
second hit in the genome might contribute the lethal phenotype. This is why
some labs clean the background of P element lines before they make the
imprecise hopout. If you can get sevearl deficiency lines or the mutant allele
for the neighboring gene, try several complementaion tests.

Check the expression pattern and functional domains for you gene, that might
help you to figure out its possible role. Making a UAS line might also helps.
It can serveral purposes. First, you can have gain of function data. Second,
tissue specific overexpression might rescue the loss of function phenotype or
you will have gain of function phenotype.

RNAi is tricky, but it is doable especially when you just works on a single
gene.

【 在 bambooo (middledoor) 的大作中提到: 】
: what I mean is that your can not publish a mutation without figuring out one
: biological aspect that causes the lethal phenotype. It is very easy to get a
: lethal mutation but it is never easy to know why the mutant flies die.
:
: 【 在 shakuras (doskey) 的大作中提到: 】
: : most phenotype can be caused by different defects.
: : Lethal is a good phenotype: easy to identify, easy to screen
: :
: : 【 在 bambooo (middledoor) 的大作中提到: 】
: : : Lethal is not a phenotype. It can be caused by so many defects. Have you
: tried
: : : mosaic analysis?
: : : 【 在 iav (damn it) 的大作中提到: 】
: : : : 问题是。我做这些就是为了把phenotype分离出来啊。再说了，致死性也是表型
。
: : :
弄了个deletion的突变，结果正好在两个基因中间，两个头对着头的基因，promoter
: : : 基因
: : : 想分
: : : 的。
: : : 片段，再转基因。一个是从基因上游-2kb到
: : : 之后
: : : 括，
: : : ---
: : : constructs都没有rescue.
: : : 有人
: : : 因）
: : : 麻烦还在于，一点rescue的迹象都没有，比如partial
: : : gene一
: :
: :
:
:


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